Small RNA assay - Agilent 2100 Bioanalyzer before miRNA microarray?

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Hui Hui
Hui Hui's picture
Small RNA assay - Agilent 2100 Bioanalyzer before miRNA microarray?

Hi,

I am just wondering is it necessary to perform small RNA assay using Small RNA kit from Agilent 2100 Bioanalyzer to characterizes the total RNA sample before performing miRNA microarray? Anyone perform miRNA microarray before? Did you characterize your sample before proceed to miRNA microarray? Anyone just use RNA 6000 Nano kit? Or need to use both RNA 600 Nano kit and also Small RNA assay?

thanks!!

-Hui- 

stylothecancer
stylothecancer's picture
Hi Hui Hui,

Hi Hui Hui,

I am not sure has you found the answer for your question or not? 

I just joined this forum and found your question. Perhaps I can try to answer your questions if they are still remains unresolved.

I am not sure which miRNA microarray you are using?

If you are using Agileny miRNA array, you just need to check your total RNA integrity with Agilent Nano 6000 kits.  From this, you also can double confirm if there is any genomic DNA contamination in your total RNA samples.

You may use Agilent small RNA assay to double check the miRNA concent in your total RNA samples, but miRNA array is  much more sensitive than small RNA assay.

Hope this would help.