space-clamp cardiomyocites

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Kiril Hristov
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space-clamp cardiomyocites

I am working on a new project and really need your help. I am studying mouse ventricular cardiomyocytes by using patch-clamp.I am trying to record sodium current in these cells. Because cardiomyocytes are large cells, there is a “space-clamp” problem which is especially important when relatively fast Na+ current is recorded. My question is how “space-clamp” problem can be addressed when Na+ current is recorded with conventional whole cell patch clamp? Should I compensate the series resistance by using “prediction correction” function? Can you please recommend protocols/articles which explain this problem in detail (particularly for cardyomyocites)? I truly appreciate your help. Thanks!