Cardiomyocyte PatchClamp

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Mendi
Mendi's picture
Cardiomyocyte PatchClamp

 Hey
Is anyone here doing patch clamp on cardiomyocytes?

I have a problem isolating viable cells. My cells are too fragmented and I barely get single cells.
I use the enzymes collagenase and Protease and my buffer has MgCl2, KCl, NaCl, HEPES, glucose, CaCl2

Please let me which protocols you use

Thank you