Raman effect in cell biology

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Sandy
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Raman effect in cell biology

Raman spectroscopy takes advantage of the fact that light beams passing through different substances will scatter in different ways, emerging with different sets of characteristic wavelengths. Such patterns can serve as fingerprints for identifying specific compounds. The Raman approach offers advantages over other technologies for single-molecule detection, in that it"s one of the most sensitive techniques available and can also be used to detect molecules in a very dilute solution of water--or potentially in the watery world of a cell. What"s more, the technique provides a way to directly observe molecules without labeling them with fluorescent tags. I was reading about raman effect and its application in biology, is there anybody currently using machines with raman effect applications. For what type of experiment?

Roshan
Roshan's picture
Sandy wrote:Raman

Sandy wrote:

Raman spectroscopy takes advantage of the fact that light beams passing through different substances will scatter in different ways, emerging with different sets of characteristic wavelengths. Such patterns can serve as fingerprints for identifying specific compounds. The Raman approach offers advantages over other technologies for single-molecule detection, in that it"s one of the most sensitive techniques available and can also be used to detect molecules in a very dilute solution of water--or potentially in the watery world of a cell. What"s more, the technique provides a way to directly observe molecules without labeling them with fluorescent tags I was reading about raman effect and its application in biology, is there anybody currently using machines with raman effect applications. For what type of experiment?

Brucker Optics offers the most advanced technology called: SENTERRA Dispersive Raman Microscope. interestingly I found Brucker's banner on the top right page of the Analytical Chemistry category.

rzheva
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There are many other

There are many other companies manufacturing dispersice Raman spectrometers and, especially, microscopes that may be usd for biocell studies. The most advanced are:
Thermo (Nicolet) Scientific (Almega, DXR Micro, etc.)
Horiba YJ (XPloRA, LabRam)
Renishaw (InVia)
Witec (Alpha 300)