No current in HEK cells

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pedro1976's picture
No current in HEK cells

 Hi, I have the problem that my HEKs stably expressing a calcium channel do not show or just show a minimum of current ( 200pA). Cells are clamped very good with a very low Leak  around -30 pA and serial resistance is usually smaller than 8 MOHM
I use 10 mM Ba2+ and it used to work perfect before. PAssage nr is 30. Any idea? Can it be a hardware problem?