Agarose gel electrophoresis

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Emma Elliot
Emma Elliot's picture
Agarose gel electrophoresis

My ladder is 100bp.I have two primers to test out. Primer Y which amplifies up to a product of 200bp and primer Z which amplifies up to 150bp fragment.I'm not very sure of how this all works. What does this mean? I thought that the ladder has to be bigger than the size of the primer. How can you measure with 100bp ladder.

Guy Sovak
Guy Sovak's picture
Ladder

YesYou need to use a bigger ladder 1K will do it. https://www.neb.com/products/n3232-1-kb-dna-ladder