substrate preparation in krabbe disease(concentration?)

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Pallavi Mishra
Pallavi Mishra's picture
substrate preparation in krabbe disease(concentration?)

Dear sir
we are doing enzyme estimation for Krabbe diease by following diggelen method. By doing so, we are facing some problem regarding substrate concentration. We would appreciate if you could provide detail procedure for preparing substrate solution . Plz help. Thank you.

yatipathak12
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