Extraction of sphingolipids

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Rina
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Extraction of sphingolipids

Can somebody advice me a protocol of sphingolipids extraction from human body fluids : blood and sputum
Thanks a lot
Rina

sidra
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in sputum and blood very

in sputum and blood very small amount of the lipid is present
 one hint from me is that to separate the components of blood
 

Rina
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Thank you very much for

Thank you very much for advice.
Did anybody used commercial kit for S1P?