Biochemistry

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Ashok Mandala
Ashok Mandala's picture
Biochemistry

Hi,I have been trying to stain fatty acid treated HepG2 cells with Oil Red O. However, I have not been successful. I could not see any staining/red color under bright field microscope. What could be the probable reason and solution?my protocol:stock 0.5g ORO in 100% isopropanol (100mL)Working solution: 3:2 ORO: DD waterafter treatment I fixed cells with 10% farmaldehyde, washed with PBS and stained with ORO for 1hr.