Seeds in Agar

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Taqi
Taqi's picture
Seeds in Agar

If i want to grow a seed in Agar that what percent of agar i have to use to grow a seed.What are the minimum requirements of nutritional media for growing a seed.

hilltrekker
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Protocols depend upon the

Protocols depend upon the kind of seeds. see the links to different types:-   
Sowing Orchid Seeds on Nutrient Media 
The Orchid Seedbank Project
PO Box 7042
Chandler, AZ 85246
http://members.cox.net/lmlauman/osp/html/sowing.html
 
GROWING TILLANDSIA SEED USING ARTIFICIAL MEDIA AND STERILE TECHNIQUES
By: Dr. Gordon Slack
http://fcbs.org/articles/slack.htm
 
 
Growth of plants in sterile conditions
http://www.biosci.ohio-state.edu/pcmb/Facilities/abrc/handling.htm
It is necessary to use sterile conditions to grow Arabidopsis for specific experiments such as selection of transformed plants, drug resistant plants, early root and shoot phenotypes, lethal mutants, etc. Otherwise, contaminants can essentially take over plant cultures. Various shapes and sizes of containers such as petri dishes, 'Magenta' boxes, or culture tubes can be used, depending on the required length of the growing time (2-3 weeks or to maturation) and characterization of phenotypes (shoot or roots). We will emphasize the use of petri dishes. All procedures should be accomplished in a sterile hood or environment.
The most commonly used media is 0.5x or 1x Murashige and Skoog (MS) mineral salts with 0.8-1% BactoAgarTM. Optional 0-3% sucrose and vitamins can be added to the media. Preparation of 0.5x MS agar media is as follows:

  1. Add 4.31 g of MS Salts to 1.8 L of distilled water and stir to dissolve.
  2. Check and adjust pH to 5.7. Adjustments can be made with 1M KOH.
  3. Dilute to final volume of 2 L and add agar (10 g / L).
  4. Autoclave 15 minutes at 15 psi, 121oC.
  5. Optional sucrose and vitamins should be added after agar media cools, before pouring solution into container (e.g. petri dishes, Magenta boxes, culture tubes).

Seeds can be surfaced sterilized by soaking for 8 min in bleach (5.25-6.15% Sodium hypochlorite) with 0.05% Tween 20 and rinsing the seeds 3-5 times with sterile, distilled water. Be sure that all bleach residue is removed. Maintain seeds in a small amount of water in a watch glass and plant immediately....................................................... see full details at:-
http://www.biosci.ohio-state.edu/pcmb/Facilities/abrc/handling.htm