Protein Native gels

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adlheras
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Protein Native gels

Hi ,

I trying to run a purified 29 KDa protein in Native gels, but always it is stuck in the wells ( when I' m not using stackig gel) or at the beguining of the separating gel. This protein is a dimer with a pI of 8.3 , i have tried to run it in native gels with pH 9.3 but it doesn' t work, it is still stuck in the wells. Does anybody knows wich could be the problem ?? 

Thank you very much !!