I trying to run a purified 29 KDa protein in Native gels, but always it is stuck in the wells ( when I' m not using stackig gel) or at the beguining of the separating gel. This protein is a dimer with a pI of 8.3 , i have tried to run it in native gels with pH 9.3 but it doesn' t work, it is still stuck in the wells. Does anybody knows wich could be the problem ??
Thank you very much !!