I would like to ask you something about a problem i face with HepG2 cells. While trying to incubate them in 24-well plates, after adding the suitable quantities of medium and cells, and move them intensively in order to be spread in the whole surface, after only 5 minutes they are amassed to the center, not to mention the next day! As a result, they form multilayers!
I would appreciate it if you could help me or send me your ideas.
Thank you very much!!