I have many quesation
1) why are we uses compontes cell BL21(DE3) for expression protein.
2) why is uses 20ºC overnight after added .1mM IPTG better than 37 ºC with 1mM IPTG for expresstion protein.
3) for monoclonal antibody :
a) how to make monoclonal
b) why did inject into mouse three times
c) what is important Freunds adjvant.