Germ cells

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msvnathan
msvnathan's picture
Germ cells

hi
i want to know the marker for germ cell isolated from testis. ie to find out whether the cells isolated by us is germ cells or not?

marcus muench
marcus muench's picture
 It might help in getting an

 It might help in getting an answer to give a little more information about what you are looking for.  Questions I have are:  What species are you working with? Are you looking for a protein to detect by flow cytometry or microscopy or are you more prone to use PCR to detect gene expression?

msvnathan
msvnathan's picture
hi

hi
Thanks for your reply. I am working on Albino Wistar rats. The rats are about 90days old, adults. i want to isolate germ cells and work on protein expression as well as mrna expression. I am planning to do flowcytometry as well as microscopic studies. If u come across some procedure please send.