expression confused

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jawad
jawad's picture
expression confused

hi,
Actually i have problem withexpression of my two genes. The first one is 14kd, i used BL21 not Bl21 (DE3) as a expression host.  i got bands in 14kd and its confirm by western blott. The second gene is 24kd, i perform expression and i got very sharp bands in 14kd also like the first gene, when i do western blott i detect these bands in same size 14kd. So now i dont know why i got 14kd bands in second gene and if these bands (first and second gene) relative to mine.
I realy need help.
Thanks,
Laith

Monu
Monu's picture
Dear Jawad

Dear Jawad

Why dont you try with 2D gel elec? May be you r problem can be solved. I will ask my other colleagues also.