Exosome Isolation from Culture Media using ExoQuick Kit

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spyder086
spyder086's picture
Exosome Isolation from Culture Media using ExoQuick Kit

Hey,

   I'm wondering whether anyone has successfully isolated exosomes from tissue culture media using Systems Bioscience ExoQuick  kit. Has anyone concentrated the media, then do the isolation to get higher end yield? From papers using this kit, most labs have successfully used this kit on serum, but not on culture media. I'm still waiting for the company tech to respond but any suggestions would be appreciated. Or does anyone use any other kits?

Thanks.

RNA-guy
RNA-guy's picture
I am also curious about this

I am also curious about this kit.  I think a lot more people would use Exoquick if the company took the time to compare their product to standard methods (e.g. ultracentrifugation) in a detailed manner.  Also, it doesn't help that no one can tell exactly what this solution is (likely some PEG formulation but who knows).  Please post back here if you have any results.  Thanks