dissection

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kipngetich
kipngetich's picture
dissection

Hi guys,
 i want to remove the salivary glands from ticks. the protocol that i am using inducates that i do it in PBS. i wonder what is the role of PBS? can someone help.

Sami Tuomivaara
Sami Tuomivaara's picture
kipngetich,

kipngetich,

PBS stands for phosphate-buffered saline, it is a common buffer used in tissue work because its pH, ionic strength and osmolarity are close to that of animal tissue fluids and serum. When you do tissue work, or excise tissue from animal to test tube, you want to bath the tissue in similar fluid that the cells are in their native environment. If you'd use buffer with pH, ionic strength or osmolarity very different from the tissue fluid, you'd risk tissue and cell damage, and hence destroying your sample. You can easily find the composition of PBS and animal tissue fluids by googling and compare them.

Cheers,