determination of total antioxidant capacity in serum

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biochemistry
biochemistry's picture
determination of total antioxidant capacity in serum

Hi,
i need a simple method for determination of total antioxidant capacity in serum samples and a method of homogenization of very hard human tissue what buffer should i use? and how can i determine it is GSH content
please help me
thanks

heehawmcduff
heehawmcduff's picture
I have previously used the

I have previously used the Trolox equivalent antioxidant capacity for assessing the antioxidant capacity of lung lavage fluid
http://www.clinchem.org/cgi/content/full/50/5/952
http://www.ncbi.nlm.nih.gov/pubmed/15769103
Promega have an assay for determining glutathione content in cells and biological fluids
http://www.promega.com/cnotes/cn022/cn022_07.htm