Cell cycle arrest in Prophase

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Biochemist986
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Cell cycle arrest in Prophase

i am working with human fibroblasts and want to arrest them in prophase. metaphase is too late! Does anyone have any ideas??
Thank you!!!!!
 

Ivan Delgado
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Hi Biochemist_986,
This is a tough one, mainly because it is so early in the cell cycle. There is an old reference to MST-16, which can arrest cells at prophase, but in all honesty I do not have experience with this. Alternative you can use IBMX (3-isobutyl-1-methylxanthine), but again I do not have experience with this.
Good luck

R Bishop
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Perhaps you can use siRNA to

Perhaps you can use siRNA to knockdown RAD51C?  Knockouts of this gene arrest in prophase.
 
Rus

heehawmcduff
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A person in my old lab used

A person in my old lab used to work on CDK (Cyclin dependent kinase) inhibitors and I think that these inhibitors can induce cell cycle arrest in prophase.