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I have recently done an experiment whereby catalase was isolated from a mixture of protein using western blotting and a antibody raised against catalase.
MY results were that it produced a single band around the 250kda mark, which sounds reasonable as the molecular weight of catalase is approx 250-300kda. However, i have been made aware that catalase contains several subunits.
i have tried to look online but have had no luck. Could someone enlighten me on the exact number of subunit that catalase has along with their respective molecular weight. Also any idea why the different subunit did not produce a various bands?