Ok, so Im only a lowly research scientist with a B.S. that reports to a phd level researcher.
Earlier this week I was asked to follow tryptophan flouresence of a protein as I titrated the protein with a ligand.
The protein has 3 Tryptophans, the ligand has 8 Tryptophans.
I am busy, and this will be a very time consuming experiment, I am trying to convince the person who asked me to do this experiment that it does not make sense due to the ligand being so Trp heavy, all we will really see is the increase in flouresence that comes from the ligand.
Am I wrong?