wana know the exact methodology of estimating superoxide dismutase activity using pyrogallol by method of marklund and marklund as modified by Nandi and chatterjee.
I have used spectrophotometric kinetic assay for superoxide dismutase activity in blood lysates. This seems to be working in my hands except the problem in preserving the sample before analysis.
Do you have the original paper of Nandi and chatterjee modification, however i have estimated SOD in blood hemolysate using Winterbaurn et.(1975) easily