the structure of the restriction enzymes

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delialpi
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the structure of the restriction enzymes

hello guys

does any 1 know the structure of the general restriction enzymes, if possible diagram show the structure.

urgent need many thanks

cfish
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Below is a link for a summary

Below is a link for a summary of the structure and function of type II restriction endonucleases. Many figures and diagrams with structures are found within this journal summary.

Link for pdf version of entire document:
http://nar.oxfordjournals.org/cgi/reprint/29/18/3705

Title: Structure and function of type II restriction endonucleases

Alfred Pingoud* and Albert Jeltsch
Institut für Biochemie (FB 08), Justus-Liebig-Universitt, Heinrich-Buff-Ring 58, D-35392 Giessen, Germany

Abstract: More than 3000 type II restriction endonucleases have been discovered. They recognize short, usually palindromic, sequences of 48 bp and, in the presence of Mg2+, cleave the DNA within or in close proximity to the recognition sequence. The orthodox type II enzymes are homodimers which recognize palindromic sites. Depending on particular features subtypes are classified. All structures of restriction enzymes show a common structural core comprising four -strands and one -helix. Furthermore, two families of enzymes can be distinguished which are structurally very similar (EcoRI-like enzymes and EcoRV-like enzymes). Like other DNA binding proteins, restriction enzymes are capable of non-specific DNA binding, which is the prerequisite for efficient target site location by facilitated diffusion. Non-specific binding usually does not involve interactions with the bases but only with the DNA backbone. In contrast, specific binding is characterized by an intimate interplay between direct (interaction with the bases) and indirect (interaction with the backbone) readout. Typically 1520 hydrogen bonds are formed between a dimeric restriction enzyme and the bases of the recognition sequence, in addition to numerous van der Waals contacts to the bases and hydrogen bonds to the backbone, which may also be water mediated. The recognition process triggers large conformational changes of the enzyme and the DNA, which lead to the activation of the catalytic centers. In many restriction enzymes the catalytic centers, one in each subunit, are represented by the PD . . . D/EXK motif, in which the two carboxylates are responsible for Mg2+ binding, the essential cofactor for the great majority of enzymes. The precise mechanism of cleavage has not yet been established for any enzyme, the main uncertainty concerns the number of Mg2+ ions directly involved in cleavage. Cleavage in the two strands usually occurs in a concerted fashion and leads to inversion of configuration at the phosphorus. The products of the reaction are DNA fragments with a 3'-OH and a 5'-phosphate.