SOD assay issues

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Lusanda Matee Matee
Lusanda Matee Matee's picture
SOD assay issues

Hi everyone, I've been trying the SOD assay on lichen material using the Beyer and Fridovich method and I'm getting higher values for the samples than the control without the enzyme extracts , these values are just the absorbtion values or OD values. I have not done an inhibition curve yet to calculate the actual value ,these values are juist as they are. Am I doing the right thing and will the values be higher once I've used the 50% inhibition formula ? by the way I'm using a spectrophotometric assay and prepared my own solutions