Problem in pyrogallol method for determination of SOD

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science love
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Problem in pyrogallol method for determination of SOD

Dear all,
I used the method of Marklund and Marklund for determination of SOD using pyrogallol in erythrocyte lysate but the results were not reproducible. What the exact method or modification could be used for solution of the problem. Is there a method for erythrocyte SOD determination using also Pyrogallol. Can anyone help solving this problem.