measurement of cytochrome C oxidase activity

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talandijnay
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measurement of cytochrome C oxidase activity

Hi All,

Could any one tell me how to calculate cytochrome c oxidase activity (nmol / min / mg)?

The slope is 0.1187, extenction coeffecient of the substrate (reduced cytochrome c)= 18.7, total volume of the reaction mixture= 1000 ul (1ml), protein concentration (enzyme conc)= 100 ug (100 microgram), amount of substrate= 42 ul (microliter), Temperature 37c, and pH 7.4.

thanx