Kd Value

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samitaarjun's picture
Kd Value

I am working on one ennzyme and I am tring to determine the Kd value. I have never work on these calcualtions beore and its the first time I am dealing with all these eqations. I need to calcualte the Kd value of my eznyme and its ligand. I know their Km, value. Can some body tell me whats the trick to calculate the Kd value from the Km. Apart from Km i know the Kcat and Vmax value as well.
I shall be thankful to you for your reply.