how to calculate the turn over numebr ?

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saransbiotech
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how to calculate the turn over numebr ?

A solution of 1% ( w/v ) starch at ph 6.7 is digested by 15 µg of β -amylase ( mol wt 152,000) .The rate of maltose
 (mol wt - 342 ) had a maximal initial velocity of 8.5 mg formed per min. Then what will be the turn over number ?

                                     dear friends kindly help me to solve this problem ...

Anuradha
Anuradha's picture
  conformation of a

  conformation of a nucleotide in DNA is affected by rotation about how many bonds conformation of a nucleotide in DNA is affected by rotation about how many bonds

Abhishek Singh
Abhishek Singh's picture
microbiology

6 bond 

Abhishek Singh
Abhishek Singh's picture
microbiology

6 bond 

Ankita Tiwari
Ankita Tiwari's picture
plz

plz gv rply of this ques

Aaditi Lele
Aaditi Lele's picture
Try using following information for calculating turnover number:

The turnover number is expressed as molecules converted per enzyme molecule active site (equivalent to moles per mole) per sec, whereas the specific activity of a pure enzyme is in units (µmoles per min) per mg enzyme. The key step is therefore to convert the mg into moles.First, remember that 1 mole contains the molecular weight (MW) in grams, hence 1 g = 1/MW moles, and1 mg = 1/(1000 x MW) molesEnzyme units are expressed in µmoles, so we need to divide the specific activity by a million to convert to moles.Now if we divide the units per mole by the number of moles we get the turnover number per min.Dividing this by 60 gives the turnover number per sec.In summary, the turnover number is calculated as:(Specific Activity/1000000) / (1/(1000 x MW)) / 60or (Specific Activity x MW) / (1000 x 60).