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biochemist major
biochemist major's picture
helping

Good evening
please i want a simple method to extract and purify horseradish peroxidase enzyme , how to assay it
regards

g a
g a's picture
Hi biochemist major
msvnathan
msvnathan's picture
hi

hi
the assay procedure was given in the following paper.
Clinical Chemistry 28: 2423-2426, 1982

Also kits are available.
http://www.biosciregister.com/Horseradish_Peroxidase_Assay_Kits/Suppliers/pid26898.htm

bye

sps
sps's picture
I  will suggest you to read

I  will suggest you to read these papers that would answer your query.
 
 
 
Ambler and Peters's paper 1984, Analytical Biochemistry 137 (1) 66-68
 
and also Molaei Rad et al., 2007 Spectrophotometric assay for horseradish peroxidase activity based on pyrocatechol–aniline coupling hydrogen donor Analytical Biochemistry Volume 362, Issue 1, 1 March 2007, Pages 38-43