Help With Gelatin Zymography

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praseet
praseet's picture
Help With Gelatin Zymography

Hello Friend,
I am currently doing Gelatin Zymography as a part of my ongoing experiments. Now here i encountered a problem and would be greatful to have your suggestions or solutions (even better). Could you please tell me how do i use markers in this technique, because at the end after staining the whole gel goes blue.
Thanking you in advance,
your student,

Braisler
Braisler's picture
I would use a Kaleidascope

I would use a Kaleidascope marker like BioRad makes. Stain and destain and you should still be able to make out the colors of 25 and 75 kDa marker which are pink instead of blue.

Good luck

saswati1
saswati1's picture
HI, I would use GELATINASE

HI, I would use GELATINASE ZYMOGRAPHY STANDARDS, from Chemicon International Inc. Catalog number CC073.

You can see the MMP standard as a clear band using this standard after staining

Alsai
Alsai's picture
Do you know any other good

Do you know any other good standards for gelatin zymography? These from Chemicon are not avaible anymore unfortunately.

rachelhauser
rachelhauser's picture
Yes, does anyone have a clue?

Yes, does anyone have a clue? I also need these standards... :(