estimation of CDNB in serum

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biochemistry
biochemistry's picture
estimation of CDNB in serum

Hi,
I am trying to estimate the amt of Glutathione-S-transferase in the serum of breast cancer patients
i have a problem in the solubility of CDNB
can you tell me about the exact method of its preparation and in what amount thanks

heehawmcduff
heehawmcduff's picture
Hopefully this paper will be

Hopefully this paper will be helpful to you:
http://www.ncbi.nlm.nih.gov/pubmed/3345272

biochemistry
biochemistry's picture
Thank you for your kind

Thank you for your kind response but This item requires a subscription* to British Journal of Anaesthesia Online.
if you have any paper about the method of preparation of CDNB in GST estimation , please send it to my e-mail or reply it to me and i will helpfull for your kind response
thanks

g a
g a's picture
CDNB can be solubilized in

CDNB can be solubilized in methanol and ethanol. you can prepare aconcentrated solution and then dilute it to a concentration not toxic to your cells.

biochemistry
biochemistry's picture
Thank you for your kind

Thank you for your kind response
I prepare a stock solution from CDNB in absolute ethanol when i dilute it a white ppt. is formed
what can i do

mohammed
mohammed's picture
I think this is unavoidable

I think this is unavoidable to get white ppt.
I tried the addition of 100mM phosphate buffer pH 6.5 to stock solution of CDNB (4mM) very slowly. a very tiny well dispersed ppt may be formed.
CDNB can be dissolved freely in ethanol and DMSO