I really need some help with this question!
The initial rate of an enzyme reaction is calculated in Absorbance/minute.
This is then converted to micromoles substrate oxidised per minute using the molar extinction coefficient of NADH which is 6.22 x 103 (i.e a molar solution of NADH in a 1cm light path has an absorbance of 6.22 x 103)
Now....Calculate the activity of the enzyme in micromole/min/mg???
Ive already converted the initial rate of the enzyme reaction to micromoles of substrate oxidised per min using the beer lambert law.....but i now cannot calculate the activity of the enzyme.
Could anyone PLEASE give a detailed explanantion of how to do it....