Enzyme

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karthibiochem
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Enzyme

Why we are usually calculating NADP/NADPH molar coefficient for most of the coupled enzyme assays?

AUen
AUen's picture
Can you expand a little bit

Can you expand a little bit more on your question?

Sakthi
Sakthi's picture
AUen wrote:Can you expand a

AUen wrote:

Can you expand a little bit more on your question?

Mostly we are using the rate of oxidation of reduced substances such as NADH/NAPDH for calculating the enzyme activity using coupled enzyme assays, why?

biolearner
biolearner's picture
Do you have a website for us

Do you have a website for us to refer? Thanks.

http://u-dna.blogspot.com
http://pcrworld.blogspot

Here are some enzyme assay protocols.
Alkaline Phosphatase

Ascorbate Oxidase

Acetyl CoA Synthase

Alanine Dehydrogenase

Alcohol Dehydrogenase

Aminolevulinate Synthase

Assay and Purification of Enzymes-Oxalate Decarboxylase; Chapter 5

Beta-galactosidase enzyme assay

Chymotrypsin

Chymotrypsinogen

Citrate Synthase

b-Galactosidase

Deoxyribonuclease

ELISA-Based Assay for Glycosyltransferases

Enzyme Assay (Presentation)

Enzyme Assay Protocol

Enzyme Assay Protocol (SudingLab)

Enzyme assay using ultra-low volume surface micromachined sensors

Gamma Glutamyl Transferase

Glucokinase

GDP-ase Assay

Glucose Oxidase

High Throughput Screening Using Enzyme Assay Microarrays

Hyaluronidase

Introduction to Enzyme Kinetics: Assay of beta-Galactosidase.

Invertase Assay

Isocitrate Lyase

Lactate Dehydrogenase

Malate Dehydrogenase

Malic Enzyme

NADH oxidase

KEX2 Assay

Kinase Enzyme Assay

Lipoxidase

Organelle Isolation and Marker Enzyme Assay (Multiple Protocols)

Peroxidase (Guaiacol Units)

Peroxidase (Purpurogallin Units)

PM ATPase Assay

Preparation of Cell Extract

PEP Carboxykinase

PEP Carboxylase

Pyruvate Carboxylase

Pyruvate Decarboxylase

Pyruvate Dehydrogenase

Pyruvate Kinase

Pyruvate Oxidase

Tyrosine Phenol Lyase

Xylitol Dehydrogenase

Xylose Reductase

Ribonuclease

SOLID-PHASE NON-SEPARATION ENZYME ASSAY

Trypsin

Trypsin Inhibitor

Trypsinogen

Wapedia - Wiki: Enzyme assay

Source:
http://ebiochemistry.blogspot.com
http://www.e-bioinfo.com