Why we are usually calculating NADP/NADPH molar coefficient for most of the coupled enzyme assays?
Can you expand a little bit more on your question?
Mostly we are using the rate of oxidation of reduced substances such as NADH/NAPDH for calculating the enzyme activity using coupled enzyme assays, why?
Do you have a website for us to refer? Thanks.
Here are some enzyme assay protocols.
Acetyl CoA Synthase
Assay and Purification of Enzymes-Oxalate Decarboxylase; Chapter 5
Beta-galactosidase enzyme assay
ELISA-Based Assay for Glycosyltransferases
Enzyme Assay (Presentation)
Enzyme Assay Protocol
Enzyme Assay Protocol (SudingLab)
Enzyme assay using ultra-low volume surface micromachined sensors
Gamma Glutamyl Transferase
High Throughput Screening Using Enzyme Assay Microarrays
Introduction to Enzyme Kinetics: Assay of beta-Galactosidase.
Kinase Enzyme Assay
Organelle Isolation and Marker Enzyme Assay (Multiple Protocols)
Peroxidase (Guaiacol Units)
Peroxidase (Purpurogallin Units)
PM ATPase Assay
Preparation of Cell Extract
Tyrosine Phenol Lyase
SOLID-PHASE NON-SEPARATION ENZYME ASSAY
Wapedia - Wiki: Enzyme assay