Electrophoresis

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NISHITA
NISHITA's picture
Electrophoresis

Hi,
   My problem is, I am not getting good resolution for Native PAGE. The protein is an enzyme, actually a cocktail of cellulase enzyme. I have tried it with 7%, 12% AND 15% gel concentration. 15% was better. But the higher molecular weight proteins are too dense plus this cocktail has too many proteins. I am using the Bio-RAD assembly and standard protocol for electrophoresis. what should I do?

Chin Fen Teo
Chin Fen Teo's picture
Have you tried a gradient gel

Have you tried a gradient gel? (says 4-15% or 4-20%, Bio-rad does carry these)

Biju
Biju's picture
 Have you considered

 Have you considered aapillary PAGE and activity staining?
Biju Joseph