cytochrome p450

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karthibiochem
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cytochrome p450

How to target and isolate mitochondrial cytochrome p450 from  mosquito?

g a
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Hi karthibiochem

Hi karthibiochem
Do you want to isolate mitochondrion from a particular genus/ species of mosquito like culex, aedes or anopheles.
The best source for the mitochondria isolation is wing muscles of the insects as they are very powerful and are very rich with the mitochondrial distribution.
I did find a number of articles on pubmed but This one may be is of more help for you
doi:10.1016/j.abb.2008.05.012   

karthibiochem
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Hi ARGERINE

Hi ARGERINE
Thank you for ur immediate reply i will try to find...
 

pawarpankaj20031
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Hi karthibiochem
If you want to isolate mitochondrial cytochrome p450 you may reffer to below link :
http://www.bioone.org/doi/abs/10.1603/0022-0493%282007%29100%5B946%3AFEOMNP%5D2.0.CO%3B2?journalCode=ecen
 

karthibiochem
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Hi pawarpankaj,

Hi pawarpankaj,
                 That link was very useful to me but i need how to isolate the particular gene??? afterthat how to check this gene??
 

Arvind Singh Pundir
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Hi karthibiochem
here are some useful links for you.....
EVOLUTION OF INSECT P450
www.biochemsoctrans.org/bst/034/1252/0341252.pdf
 
The Insect P450 Site   -   A Resource For Research On Insect Cytochromes P450
p450.sophia.inra.fr/     
CYTOCHROME P450 HOMEPAGE                                                             drnelson.utmem.edu/CytochromeP450.html