How to target and isolate mitochondrial cytochrome p450 from mosquito?
Do you want to isolate mitochondrion from a particular genus/ species of mosquito like culex, aedes or anopheles.
The best source for the mitochondria isolation is wing muscles of the insects as they are very powerful and are very rich with the mitochondrial distribution.
I did find a number of articles on pubmed but This one may be is of more help for youdoi:10.1016/j.abb.2008.05.012
Thank you for ur immediate reply i will try to find...
If you want to isolate mitochondrial cytochrome p450 you may reffer to below link :http://www.bioone.org/doi/abs/10.1603/0022-0493%282007%29100%5B946%3AFEOMNP%5D2.0.CO%3B2?journalCode=ecen
That link was very useful to me but i need how to isolate the particular gene??? afterthat how to check this gene??
here are some useful links for you.....EVOLUTION OF INSECT P450www.biochemsoctrans.org/bst/034/1252/0341252.pdf
The Insect P450 Site - A Resource For Research On Insect Cytochromes P450p450.sophia.inra.fr/ CYTOCHROME P450 HOMEPAGE drnelson.utmem.edu/CytochromeP450.html