colourimetry method for assaying tyrosinase activity

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r.m.sh
r.m.sh's picture
colourimetry method for assaying tyrosinase activity

please help me to find method for assaying tyrosinase activity colourimetry
regards
 

Arvind Singh Pundir
Arvind Singh Pundir's picture
refer Polyphenol Oxidase

refer
Polyphenol Oxidase Assay(tyrosinase)
www.worthington-biochem.com/TY/assay.html
Kinetic Analysis of the Enzyme Tyrosinase
darwin.nmsu.edu/~blyons/BCHE_397/tyrosinase.htm

hilltrekker
hilltrekker's picture
Hi

Hi
have a look at this article,
Possible involvement of proteolytic degradation of tyrosinase in the regulatory effect of fatty acids on melanogenesis
Hideya Andoa,b,c, Yoko Funasakaa, Masahiro Okaa, Akiko Ohashia, Minao Furumurab, Jun Matsunagab, Naoko Matsunagab, Vincent J. Hearingb, and Masamitsu Ichihashia
a Department of Dermatology, Kobe University School of Medicine, Kobe, Japan
b Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD
c Central Research Institute, SUNSTAR Inc., Takatsuki, Osaka, Japan
http://www.jlr.org/cgi/content/full/40/7/1312
It also contains info on tyrosine assay. hope it helps.