I would need your help and all suggestions for doing a kinase assay in-vitro to detect the activity of CDK6. I have never done a kinase assay and as I see the papers published, they have all used only radioactive detection method!!. It would be of great help if anyone around has had experience in kinase assay preferably in looking for the CDK6 or similar kinase activities.
all help and suggestions are highly appreciated and I would be deeply grateful for them if a protocol and also if possible a source from where to get the reagents can help a lot more..
thanking you all for your time and help
best of things