I have an enzyme from a hyperthermophile, I wanted to do the assay
of this protein. I have read that people incubate enzyme at different
temperatures and different times and do the assays for these at room
temperature itself. Suppose this protein is not at all active at room
temperature what should I do?
also the I need to use a coupled assay, so if I need to do the assay
at high temperature, the coupling enzymes wont support?
Can anyone give a possible soulution?