advice for practical enzyme kinetics experiments

3 posts / 0 new
Last post
mohammed
mohammed's picture
advice for practical enzyme kinetics experiments

Sallam,
I am doing my enzyme kinetics with alot of mistakes. so I am doing a single experiment 10Xs times till find the cause.
I would like to have a textbook or manual or any thing which can help me to directly trouble shoot the errors when it appears
I am working with glutathione-s-transferase enzyme.
It is urgent.
Thanks

Janet Gonzalez
Janet Gonzalez's picture
extinction coefficient

I have an old spectrophotometer in the lab and my enzyme Ab max = 486 nm but the machine has only a 450 nm filter.  Is there a calculation that I can use to convert from one wavelength to another?

Aaditi Lele
Aaditi Lele's picture
Enzyme kinetics

You can refer to this page for basic information : https://chem.libretexts.org/Core/Biological_Chemistry/Catalysts/Enzymati...For detailed information you could read this: http://www.springer.com/us/book/9781402007286