Quantifying Chemiluminescence using image J

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montgomm
montgomm's picture
Quantifying Chemiluminescence using image J

Which plug-in or what function of image J do people use to quantify their bands from in western blots?

I've been using the "Analyze/Gel" function, but not sure that is actually the correct way to go.

Suggestions?

lscraig
lscraig's picture
you use a mac or pc?

you use a mac or pc?

montgomm
montgomm's picture
I use PC

I use PC

I found out the best way is to use the Ctrl +M "Measure function" for each band and in the measurement options select "Integrated Density"

This has worked very well since I implemented it.

Guy Sovak
Guy Sovak's picture
Any one got an old version

Any one got an old version for an old PC.
The one that I downloaded from the site needs a Mac OSX I got Mac OS IX
Guy

vanishing
vanishing's picture
you could also consider this

you could also consider this alternatives

Gelscape
http://www.gelscape.ualberta.ca:8080/htm/index.html

(no experience with that I must admit)

qiang wang
qiang wang's picture
for the Ctrl + M, you will

for the Ctrl + M, you will need first do the color invert

christiandersen
christiandersen's picture
You can check out www

You can check out www.quantifyimage.com if your using windows and need a commercial tool which includes support.

Sushma Dagar
Sushma Dagar's picture
Hi montgomm,

Hi montgomm,
 Recently I came throught this post.
You don't need a separate image J plug-in for quantifying your western blot bands. I have also been using same "Analyze/Gel" option and its correct. It does not make much difference whether you are using windows or mac version of image J. I have tried both the ways.