autosensitivity

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Rajeshwari patel
Rajeshwari patel's picture
autosensitivity

Dear all,
I want to know that  in the floruracence and luminance why it is always measure in terms of Relative means relative luminance and relative fluoresce .It is relative to what? Instrument??, lamp??, plate ??? Standard??, blank? Or control??
Thanks
Rajeshwari
 

Ivan Delgado
Ivan Delgado's picture
 

 
Hi Rajeshwari,
Fluorescence and luminescence are typically measured in relative terms because the signal you see is not the raw signal but a measure that has been normalized against something (hence "relative"). The "relative" component is dependent on experimental setup. It could be relative to a no-substrate control, a no-enzyme control, etc. Knowing the kind of experiment you are running and the instrument (and software) you are using, you can determine what "relative" means. It does not mean the same thing in every case since each system may normalize the signal in a different manner.

Shubhangi
Shubhangi's picture
Hi

Hi
In connection to the discussion :
since different instruments/ systems have different adjustmets for normalization, can someone suggest how to compare readings/results  from different instruments?
 

Ivan Delgado
Ivan Delgado's picture
 

 
I can't really give you a good answer to this. In my experience the only way you can compare readings obtained from one instrument to those obtained from another instrument is to run the same standards in both instruments and then normalize the results based on the ratio/constant obtained by comparing the standards. While this is straight forward to do, it requires you to run standards in every plate. As a result it may be better to simply design the experiment in such a way as not to have to compare results between instruments (i.e. have all pertinent samples/experiments to be compared together in one plate).
 
Shubhangi wrote:

Hi
In connection to the discussion :
since different instruments/ systems have different adjustmets for normalization, can someone suggest how to compare readings/results  from different instruments?