HI ANYBODY IS THERE
Is there a specific question you would like to ask? I assume you are interested in superoxide dismutase activity in yeast. Can you be more specific? What species are you working with? etc.
hi tony please see your inbox for message. well i am working on the pentose utilizing yeast pachysylon tennophilus i will work on the sod activity also how we can measure the sod activity in the yeast cells.
Here is a reference for SOD activity that you might find useful:
Worthington Biochemical Corporation - Superoxidase Dismutase
Activity of SOD has been determined in two ways:
1. Inhibition by the enzyme of an O2- dependent reaction.
2. Pulse radiolytic methods (Rigo et al. 1975)
See: Beauchamp and Fridovich (1971);
Misra and Fridovich (1972);
Method: The method employed at Worthington is essentially that of Winterbourn et al. (1975) and is based on the ability of superoxide dismutase to inhibit the reduction of nitro-blue tetrazolium by superoxide. One unit is defined as that amount of enzyme causing half the maximum inhibition of NBT reduction. The reaction velocity will depend largely on somewhat variable assay conditions such as light intensity and reaction temperature. Calibration of the method in individual laboratories is recommended.
THAN YOU VERY MUCH DEAR TONY I WILL FOLLOW YOUR ADVISE. I AM SORRY THAT YOU WERE VERY BUSY AND DISTRUBED BY ME IT IS REALLY VERY TIDIOUS JOB TO HANDLE THE POST ON WHICH YOU ARE.
ALL THE BEST FOR IT. AND THANKS AGAIN
No problem at all! It's my pleasure to help!
I would only ask you to help out the rest of the board by posting if this method works for your applications.