what's the best concentration of MG132

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graduate
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what's the best concentration of MG132

Hello,
I'm doing an experiment where I have to inhibit the proteosome degradation and measure Nrf2 accumulation in the Caco-2 cells. My question is, what is the best concentration of MG132 to use. If I do a dose response curve, should I use the IC50 value or the maximum value as the concentration of MG132?

Thanks