Hi, i have a question. I am trying to do western using an antibody and ahve tried the recommended concentration. I get a bright yellow band across the gel. The band does not correspond to the molecular weight of the protein i want to detect. It is bright yellow and when i develop the blot it is like it is burned out at that spot. It doesn't have to do with exposure since the band appears after incubation with the primary antibody.....
I have thought it maybe a non specific reaction but the color of the band is strange. Why would it appear so bright?
Does anyone have any ideas?