treating dialysis tube with EDTA

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yosefpour
yosefpour's picture
treating dialysis tube with EDTA

I treated my dialysis bag(tube) with boiling distillled water for 35 min and used it for purification of my protein from histide and sorbate impurities. now I have found out that dialysis bag(tube) needs to be treated with EDTA to remove heavy metals from it.

I wonder how and to what extent this may affect my experiments? any idea about the amount of heavy metals in dialysis bag? any way to detect the presence of this heavy metals in the dialyzed solution?

anyway to removw this heavy metals from the dialyzed solution?

Jason King
Jason King's picture
EDTA will bind di-valent

EDTA will bind di-valent cations (not just heavy metals). Removing these may prevent your proteins from binding to the dialysis membrane and being lost.

If the manufacturer thought that heavy metals were present in the tubing and that this would prevent the efficient functioning of his product - wouldn't he have already treated the tubing to prevent this? The metal ions will be in the extract or protein sample you are trying to clean up.