Sequence specific pulldown of dsDNA

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K-dude
K-dude's picture
Sequence specific pulldown of dsDNA

 Hi!

Is it possible to use biotin-conjugated DNA oligos and streptavidin beads to sequence-specifically pulldown double stranded genomic DNA or can that only be done on single stranded DNA?
If it's not possible, are there any other ways of doing it?
 
I want to do "reverse ChIP", i.e. I want to pulldown a specific stretch of double stranded genomic DNA and then use western blot to see if my protein of interest was pulled down with the DNA. 
 
Cheers!
Bacon
 

Chin Fen Teo
Chin Fen Teo's picture
Hi Baconman,

Hi Baconman,

In theory, it should work. I guess it is just a matter of optimization, which requires patience of a saint! :-)

Good luck.