Reagents that are not compatible with BCA

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Guy Sovak
Guy Sovak's picture
Reagents that are not compatible with BCA

Hi all,
I have a problem with BCA protein concentration assay.
I am using a flow through from a column.
The washing buffer is not the "problem maker" something else is.
Did anyone had also problems with flow through protein concentration tested by BCA?
Guy

bwbrian
bwbrian's picture
Reducing agents are generally

Reducing agents are generally the culprit when BCA's don't work out. Pierce has a tip on how to remove these substances here:

http://www.piercenet.com/files/TR0008dh5-TCA-acetone-precip.pdf