Protein Purification

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algalvan
algalvan's picture
Protein Purification

I am protein purifying and I accidentally used DNA sample buffer to quench my lysates instead of using SDS sample buffer. Does anyone have advise?

swannnyy
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Add SDS to 2%, and DTT 0.8%

Add SDS to 2%, and DTT 0.8% and you should be right.