PEGylation

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srisailam
srisailam's picture
PEGylation

Hello members,
I have a protein that was pegylated. I need to analyze the protein in MS. So PEGylation will be an issue in MS. So, I would like to remove the tag or digest the tag to make it very short. Is there any assays that do this or any kits?
Thank you very much for your suggestons
sailam

qiang wang
qiang wang's picture
I found this article about

I found this article about acid-catalyzed dePEGylation strategy may be helpful

http://pubs.acs.org/cgi-bin/article.cgi/langd5/2003/19/i16/pdf/la030104y.pdf

Boomer et. al.
Acid-Triggered Release from Sterically Stabilized Fusogenic Liposomes via a Hydrolytic DePEGylation Strategy
Langmuir, 19 (16), 6408 -6415, 2003.

srisailam
srisailam's picture
thank you very much. I dont

thank you very much. I dont know how my protein behave with the acid treatment. anyways I will look into the article. Thank you very much for your suggestions.
sailam