Insulin precipitation

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g a
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Insulin precipitation

Hi all
I need to check the amount of insulin produced by the cultured cells. Has anyone done that before......
Kindly help me in locating a protocol that can be used for the precipitation of Insulin from cells or cell culture supernatant.
I dont think orgenic solvent like acetone can be used for that due to relatively lower molecular weight of Insuln.

g a
g a's picture
To add to the query..... Does

To add to the query..... Does anyone knows how is Insulin purified during recombinant insulin production ...? Is it affinity purified or precipitated?